Journal:

Article Title: Beta-4 tubulin identifies a primitive cell source for oligodendrocytes in the mammalian brain

doi: 10.1523/JNEUROSCI.1027-09.2009

Figure Lengend Snippet: βT4 cells in the subventricular zone of the adult human brain do not express markers specific for characterized central nervous system cells. βT4 cells (A, B, β4 tubulin, green, arrows) do not express markers specific for microglia (A, Iba1, red, arrowheads) or oligodendrocytes (B, PLP, red, arrowheads). In serially cut sections stained for individual antigens, the morphology and distribution of βT4 cells (C, arrows) are distinct from NG2-positive oligodendrocyte progenitor cells (D, arrows) or PSA-NCAM-positive committed neuronal progenitor cells (E, arrows). Ven = Lateral Ventricle. Scale bars: A,B, 20 μm; C-E, 50 μm.

Article Snippet: Antibodies and dilutions used on tissue sections were: Class IV beta tubulin (1:10000, Sigma, St. Louis, MO), Tuj1 (1:1000, Covance, Princeton, NJ), GFAP (1:10000, Dako, Glostrup, Denmark), Iba1 (1:4000, Gift from Dr. Shinichi Kohsaka), NG2 (1:250, Chemicon International, Temecula, CA), PLP (1:400, Agmed, Bedford, MA), PSA-NCAM (1:200, Pharmingen, San Diego, CA), goat anti-rat IgG biotinylated (1:1000, Vector Laboratories, Burlingame, CA), goat anti-mouse IgG biotinylated (1:1000, Vector Laboratories), goat anti-mouse IgG Alexa (1:1000, Invitrogen, Carlsbad, CA), goat anti-rabbit FITC (1:1000, Invitrogen), goat anti-mouse IgG1 FITC (1:1000, Invitrogen), goat anti-mouse IgG2a Alexa (1:1000, Invitrogen), goat anti-rabbit IgG Alexa (1:1000, Invitrogen), goat anti-rabbit IgG biotinylated (1:1000, Vector Laboratories), and goat anti-mouse IgM biotinylated (1:1000,Vector Laboratories).

Techniques: Staining

Journal: The Saudi Dental Journal

Article Title: Conditioned-medium of stem cells from human exfoliated deciduous teeth prevent apoptosis of neural progenitors

doi: 10.1016/j.sdentj.2022.08.005

Figure Lengend Snippet: Flow cytometry identification of neuron progenitor phenotypes with polysialic acid neural cell adhesion molecule ( PSA–NCAM) + A2B5 - markers. Purity was 95.86 %.

Article Snippet: Neural progenitors were characterized using antibodies against A2B5, the marker for immature glial-committed precursors (130093582; Miltenyi Biotec, Inc., Bergisch Gladbach, Germany), and against PSA-NCAM (130–120-437; Miltenyi Biotec, Inc.).

Techniques: Flow Cytometry

Journal: Theranostics

Article Title: Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtype

doi: 10.7150/thno.96163

Figure Lengend Snippet: The molecular and clinical characteristics of LP subtype breast cancer. (A) Stacked bar plot showing the deconvolution result of breast tumors from the METABRIC cohort. Colors of the bars denote three cell lineages as shown in the legend. The y axis stands for the proportion of each cell lineage in a given bulk tumor sample. Within the x axis, each column represents one tumor case. The annotation bar above denotes the molecular subtypes of bulk tumors that are defined by the dominant cell lineage within each tumor, where yellow represents the mixture of multiple cell lineages. (B) Kaplan-Meier plot showing worse clinical outcome in LP subtype patients within the METABRIC cohort. P value is calculated using the log-rank test. (C) Spearman correlation between LP score and diverse mutational signatures. Correlations with P < 0.05 are marked with an asterisk. (D) Violin plot comparing the HRD score between LP-low and LP-high breast tumors in TCGA. Unpaired two-sided Wilcoxon test. (E) Violin plot comparing the expression level of LP score of breast tumors with different responses to NAC treatment. Unpaired two-sided Wilcoxon test. (F) Violin plot comparing the expression level of LP score of breast tumors with different responses to PARP inhibitor treatment in the I-SPY2 cohort. Unpaired two-sided Wilcoxon test. (G) Bubble heatmap showing up-regulated pathways enriched in breast tumors with high LP proportion via GSEA. Dot size indicates the normalized enrichment score (NES), colored based on the adjusted P value. (H) Violin plot comparing the T cell exhausted signature score between LP-low and LP-high breast tumors in TCGA. Unpaired two-sided Wilcoxon test. (I) Violin plot comparing the expression level of LP score of breast cancer patients with different responses to anti-PD1 treatment. Unpaired two-sided Wilcoxon test. (J) Immunohistochemistry of breast tissue microarray for PSAT1, ER and CK14. (K) LP subtype of breast cancer patients with PSAT1 high /ER low /CK14 low were associated with poor DFS. P value is calculated using the log-rank test.

Article Snippet: Six series of tissue microarray were used to detect PSAT1 (Proteintech, 10501-1-AP, 1:200), ER (Proteintech, 21244-1-AP, 1:200), CK14 (Servicebio, GB11803, 1:200), CKB (Proteintech, 66714-1-IG, 1:500) and CA4 (Proteintech, 13931-1-AP, 1:200).

Techniques: Expressing, Immunohistochemistry, Microarray